Objective
Not currently seeking employment. Hired for HX research with Genentech effective December 16, 2013.
Experience
University of Pennsylvania Perelman School of Medicine, Philadelphia,
PA
08/2007-11/2013, Ph.D. research.
Department of Biochemistry and Molecular Biophysics & Biomedical Graduate Studies.
Mentored by Professor S. Walter Englander.
Dissertation Title: The application of hydrogen exchange to study large protein folding & structural dynamics.
NC State University Dept. of Molecular & Structural Biochemistry • Raleigh, NC • 05/2006 – 6/2007. Biochemistry Research Technician supervised by Professors Michael Goshe & Steve Clouse.
NC State University Genetics & Zoology Departments, Raleigh, NC, 05/2005 – 05/2006. Molecular Biology Research Technician supervised by Professor Robert R. H. Anholt.
08/2007-11/2013, Ph.D. research.
Department of Biochemistry and Molecular Biophysics & Biomedical Graduate Studies.
Mentored by Professor S. Walter Englander.
Dissertation Title: The application of hydrogen exchange to study large protein folding & structural dynamics.
- Determination of the protein folding pathway (HX
MS) and native structure stability (NMR) of the largest protein studied to date
at structural resolution using experiments designed specifically for this task
(publication 1).
- Development of algorithmic strategies to
determine site-resolved deuterium occupancy from peptide fragment level HX MS
data (publication 2).
- Development of hydrogen exchange mass
spectrometry (HX MS) experiments designed to study protein folding. My work
here involves experimental optimization (publications
3-5) and algorithms designed to process and interpret HX MS experimental
results.
- Nuclear Magnetic Resonance Spectroscopy (NMR):
substantial contributions to hydrogen exchange methodologies by the development
of a multidimensional sparse-sampling NMR experiment with the ability to study
native state hydrogen exchange for the first time in large (>40kDa) proteins
at amino acid resolution. I also developed algorithms needed to process the
data using multidimensional Fourier transformation and signal analysis. (publication 6).
- Self-taught computer programming in the Python
language to create algorithms for processing and analysis of data from hydrogen
exchange experiments by NMR and MS.
- Presentation of research at three technical
conferences with 200+ attending professionals in addition to numerous on-campus
presentations.
- Teaching assistant for Macromolecular Biophysics
II (BMB 519), Spring Semester, 2010. Responsibilities include weekly office
hours, formal classroom sessions for material review, and grading of
coursework.
NC State University Dept. of Molecular & Structural Biochemistry • Raleigh, NC • 05/2006 – 6/2007. Biochemistry Research Technician supervised by Professors Michael Goshe & Steve Clouse.
- Development of multi-step protein purification
of integral membrane proteins.
- Proteomic
analysis of post-translational modifications on integral membrane proteins by
LC-MS.
- Development
of novel protease removal strategies minimizing sample loss (publication 7).
NC State University Genetics & Zoology Departments, Raleigh, NC, 05/2005 – 05/2006. Molecular Biology Research Technician supervised by Professor Robert R. H. Anholt.
- Gene sequencing
and ANCOVA analysis of identified single nucleotide polymorphisms in genes
regulating olfaction with behavioral assays in D. melanogaster.
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