Benjamin T. Walters
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Objective

Not currently seeking employment.  Hired for HX research with Genentech effective December 16, 2013. 

Experience

University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 
08/2007-11/2013, Ph.D. research. 
Department of Biochemistry and Molecular Biophysics & Biomedical Graduate Studies.
Mentored by Professor S. Walter Englander.
Dissertation Title: The application of hydrogen exchange to study large protein folding & structural dynamics.

  • Determination of the protein folding pathway (HX MS) and native structure stability (NMR) of the largest protein studied to date at structural resolution using experiments designed specifically for this task (publication 1).
  • Development of algorithmic strategies to determine site-resolved deuterium occupancy from peptide fragment level HX MS data (publication 2).
  • Development of hydrogen exchange mass spectrometry (HX MS) experiments designed to study protein folding. My work here involves experimental optimization (publications 3-5) and algorithms designed to process and interpret HX MS experimental results.
  • Nuclear Magnetic Resonance Spectroscopy (NMR): substantial contributions to hydrogen exchange methodologies by the development of a multidimensional sparse-sampling NMR experiment with the ability to study native state hydrogen exchange for the first time in large (>40kDa) proteins at amino acid resolution. I also developed algorithms needed to process the data using multidimensional Fourier transformation and signal analysis. (publication 6).
  • Self-taught computer programming in the Python language to create algorithms for processing and analysis of data from hydrogen exchange experiments by NMR and MS.
  • Presentation of research at three technical conferences with 200+ attending professionals in addition to numerous on-campus presentations.
  • Teaching assistant for Macromolecular Biophysics II (BMB 519), Spring Semester, 2010. Responsibilities include weekly office hours, formal classroom sessions for material review, and grading of coursework.

NC State University Dept. of Molecular & Structural Biochemistry • Raleigh, NC • 05/2006 – 6/2007.  Biochemistry Research Technician supervised by Professors Michael Goshe & Steve Clouse.
  • Development of multi-step protein purification of integral membrane proteins.
  • Proteomic analysis of post-translational modifications on integral membrane proteins by LC-MS.
  • Development of novel protease removal strategies minimizing sample loss (publication 7).

NC State University Genetics & Zoology Departments, Raleigh, NC, 05/2005 – 05/2006.  Molecular Biology Research Technician supervised by Professor Robert R. H. Anholt.
  • Gene sequencing and ANCOVA analysis of identified single nucleotide           polymorphisms in genes regulating olfaction with behavioral assays in D. melanogaster.


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